Selective enrichment of azide-containing peptides from complex mixtures

J Proteome Res. 2009 Jul;8(7):3702-11. doi: 10.1021/pr900257z.

Abstract

A general method is described to sequester peptides containing azides from complex peptide mixtures, aimed at facilitating mass spectrometric analysis to study different aspects of proteome dynamics. The enrichment method is based on covalent capture of azide-containing peptides by the azide-reactive cyclooctyne (ARCO) resin and is demonstrated for two different applications. Enrichment of peptides derived from cytochrome c treated with the azide-containing cross-linker bis(succinimidyl)-3-azidomethyl glutarate (BAMG) shows several cross-link containing peptides. Sequestration of peptides derived from an Escherichia coli proteome, pulse labeled with the bio-orthogonal amino acid azidohomoalanine as substitute for methionine, allows identification of numerous newly synthesized proteins. Furthermore, the method is found to be very specific, as after enrichment over 87% of all peptides contain (modified) azidohomoalanine.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alanine / analogs & derivatives
  • Alanine / chemistry
  • Amino Acid Sequence
  • Azides / chemistry*
  • Cations
  • Chromatography, Ion Exchange / methods
  • Cross-Linking Reagents / pharmacology
  • Cytochromes c / chemistry
  • Glutarates / chemistry
  • Kinetics
  • Mass Spectrometry / methods
  • Methionine / chemistry
  • Molecular Sequence Data
  • Peptides / chemistry*
  • Proteome
  • Proteomics / methods

Substances

  • Azides
  • Cations
  • Cross-Linking Reagents
  • Glutarates
  • Peptides
  • Proteome
  • azidohomoalanine
  • Cytochromes c
  • Methionine
  • Alanine