Hunting for the 5th base: Techniques for analyzing DNA methylation

Biochim Biophys Acta. 2009 Sep;1790(9):847-62. doi: 10.1016/j.bbagen.2009.02.001. Epub 2009 Feb 9.

Abstract

Background: In the recent past large progress has been made in the analysis of the epigenome, the entirety of epigenetic modifications, and its meaning for the implementation of the genetic code. Besides histone modifications and miRNA expression, DNA methylation is one of the key players in the field of epigenetics, involved in numerous regulatory processes.

Methods: In the present review we focus on methods for the analysis of DNA methylation patterns and present an overview about techniques and basic principles available for this purpose.

Results and general significance: We here discuss advantages and disadvantages of various methods and their feasibility for specific tasks of DNA methylation analysis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Algorithms
  • Animals
  • CpG Islands
  • DNA Methylation*
  • Epigenesis, Genetic*
  • Humans
  • Oligonucleotide Array Sequence Analysis
  • Polymerase Chain Reaction