Scale-up of counter-current chromatography: demonstration of predictable isocratic and quasi-continuous operating modes from the test tube to pilot/process scale

J Chromatogr A. 2009 Dec 11;1216(50):8787-92. doi: 10.1016/j.chroma.2009.03.040. Epub 2009 Mar 19.

Abstract

Predictable scale-up from test tube derived distribution ratios and analytical-scale sample loading optimisation is demonstrated using a model sample system of benzyl alcohol and p-cresol in a heptane:ethyl acetate:methanol:water phase system with the new 18 L Maxi counter-current chromatography centrifuge. The versatility of having a liquid stationary phase with its high loading capacity and flexible operating modes is demonstrated at two different scales by separating and concentrating target compounds using a mixture of caffeine, vanillin, naringenin and carvone using a quasi-continuous technique called intermittent counter-current extraction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzyl Alcohol / isolation & purification
  • Centrifugation
  • Countercurrent Distribution / instrumentation*
  • Cresols / isolation & purification
  • Pilot Projects

Substances

  • Cresols
  • 4-cresol
  • Benzyl Alcohol