A novel co-polymer based on hydroxypropyl alpha-cyclodextrin conjugated to low molecular weight polyethylenimine as an in vitro gene delivery vector

Hongliang Huang; Hai Yu; Da Li; Yang Liu; Fenping Shen; Jun Zhou; Qingqing Wang; Guping Tang

doi:10.3390/ijms9112278

A novel co-polymer based on hydroxypropyl alpha-cyclodextrin conjugated to low molecular weight polyethylenimine as an in vitro gene delivery vector

Int J Mol Sci. 2008 Nov;9(11):2278-2289. doi: 10.3390/ijms9112278. Epub 2008 Nov 21.

Authors

Hongliang Huang¹, Hai Yu², Da Li², Yang Liu², Fenping Shen², Jun Zhou³, Qingqing Wang², Guping Tang³

Affiliations

¹ School of Life Science, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou, P. R. China.
² Institute of Immunology, Zhejiang University, Yuhangtang Road 388, Hangzhou, P. R. China.
³ Institute of Chemical Biology and Pharmaceutical Chemistry, Zhejiang University, Tianmushan Road 148, Hangzhou, P. R. China.

Abstract

A novel co-polymer based on 2-hydroxypropyl-alpha-cyclodextrin cross-linked by low molecular weight polyethylenimine was synthesized as a gene delivery vector. The copolymer could bind and condense DNA tightly. It showed lower cytotoxicity than PEI 25kDa in SK-BR-3 cells. Transfection efficiency was increased over 5.5-fold higher than PEI 25 kDa in SK-BR-3 cells in complete serum medium. It is a potential candidate vector for gene therapy.

Keywords: 2-hydroxypropyl-α-cyclodextrin; Polyethylenimine; co-polymer; non-viral gene delivery vector.