Antimicrobial susceptibility of Mycoplasma pneumoniae isolates and molecular analysis of macrolide-resistant strains from Shanghai, China

Antimicrob Agents Chemother. 2009 May;53(5):2160-2. doi: 10.1128/AAC.01684-08. Epub 2009 Mar 9.

Abstract

Fifty-three Mycoplasma pneumoniae strains were isolated from pediatric patients in Shanghai, China, from October 2005 to February 2008. Of 53 clinical isolates, 44 (83%) were resistant to erythromycin (MICs of >128 microg/ml for all 44 strains), azithromycin, and clarithromycin. All macrolide-resistant M. pneumoniae strains harbored an A-to-G transition mutation at position 2063 in 23S rRNA genes. Forty-five (85%) clinical isolates were classified into the P1 gene restriction fragment length polymorphism type I, and six (11%) were type II.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adhesins, Bacterial / genetics
  • Anti-Bacterial Agents / pharmacology*
  • Child
  • China
  • Drug Resistance, Bacterial / genetics*
  • Genes, rRNA
  • Humans
  • Macrolides / pharmacology*
  • Microbial Sensitivity Tests
  • Mutation
  • Mycoplasma pneumoniae / classification
  • Mycoplasma pneumoniae / drug effects*
  • Mycoplasma pneumoniae / genetics
  • Mycoplasma pneumoniae / isolation & purification
  • Pneumonia, Mycoplasma / microbiology
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • RNA, Ribosomal, 23S / genetics
  • Respiratory Tract Infections / microbiology*
  • Sequence Analysis, DNA

Substances

  • Adhesins, Bacterial
  • Anti-Bacterial Agents
  • Macrolides
  • RNA, Ribosomal, 23S
  • adhesin, Mycoplasma pneumoniae