Detection of galectin-3 in patients with inflammatory bowel diseases: new serum marker of active forms of IBD?

Inflamm Res. 2009 Aug;58(8):503-12. doi: 10.1007/s00011-009-0016-8. Epub 2009 Mar 7.

Abstract

Objective: It is an open question whether multifunctional galectin-3 can be a serum marker in inflammatory bowel disease.

Methods: Western blots and commercial ELISA detected and quantitated the lectin immunocytochemistry using double labeling localized it in tissue sections.

Results: Serum concentrations were significantly increased in specimen of patients with active and remission-stage ulcerative colitis and Crohn's disease, associated with emerging positivity of CD14(+) cells.

Conclusion: Enhanced concentration of galectin-3 in serum reflects presence of disease and points to its involvement in the pathogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomarkers
  • Blotting, Western
  • Colitis / chemically induced
  • Colitis, Ulcerative / blood
  • Colon / metabolism
  • Crohn Disease / blood
  • Dextran Sulfate
  • Electrophoresis, Polyacrylamide Gel
  • Escherichia coli / metabolism
  • Female
  • Fluorescein-5-isothiocyanate
  • Fluorescent Dyes
  • Galectin 3 / blood*
  • Humans
  • Immunohistochemistry
  • Inflammatory Bowel Diseases / blood*
  • Inflammatory Bowel Diseases / chemically induced
  • Inflammatory Bowel Diseases / diagnosis
  • Lectins / metabolism
  • Lipopolysaccharide Receptors / analysis
  • Lipopolysaccharide Receptors / metabolism
  • Mice
  • Mice, Inbred BALB C

Substances

  • Biomarkers
  • Fluorescent Dyes
  • Galectin 3
  • Lectins
  • Lipopolysaccharide Receptors
  • Dextran Sulfate
  • Fluorescein-5-isothiocyanate