Plasma cytokines and chemokines in primary graft dysfunction post-lung transplantation

Am J Transplant. 2009 Feb;9(2):389-96. doi: 10.1111/j.1600-6143.2008.02497.x. Epub 2008 Dec 15.

Abstract

Primary graft dysfunction (PGD) after lung transplantation causes significant morbidity and mortality. We aimed to determine the role of cytokines and chemokines in PGD. This is a multicenter case-control study of PGD in humans. A Luminex analysis was performed to determine plasma levels of 25 chemokines and cytokines before and at 6, 24, 48 and 72 h following allograft reperfusion in 25 cases (grade 3 PGD) and 25 controls (grade 0 PGD). Biomarker profiles were evaluated using a multivariable logistic regression and generalized estimating equations. PGD cases had higher levels of monocyte chemotactic protein-1 (MCP-1)/chemokine CC motif ligand 2 (CCL2) and interferon (IFN)-inducible protein (IP-10)/chemokine CXC motif ligand 10 (CXCL10) (both p < 0.05), suggesting recruitment of monocytes and effector T cells in PGD. In addition, PGD cases had lower levels of interleukin (IL-13) (p = 0.05) and higher levels of IL-2R (p = 0.05). Proinflammatory cytokines, including tumor necrosis factor (TNF)-alpha, and IFN-gamma decreased to very low levels after transplant in both PGD cases and controls, exhibiting no differences between the two groups. These findings were independent of clinical variables including diagnosis in multivariable analyses, but may be affected by cardiopulmonary bypass. Profound injury in clinical PGD is distinguished by the upregulation of selected chemokine pathways, which may useful for the prediction or early detection of PGD if confirmed in future studies.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Adult
  • Biomarkers / blood*
  • Case-Control Studies
  • Chemokines / blood*
  • Cohort Studies
  • Cytokines / blood*
  • Female
  • Graft Rejection
  • Humans
  • Inflammation Mediators
  • Lung Transplantation / adverse effects*
  • Male
  • Middle Aged
  • Primary Graft Dysfunction / blood
  • Primary Graft Dysfunction / etiology*
  • Prospective Studies
  • Young Adult

Substances

  • Biomarkers
  • Chemokines
  • Cytokines
  • Inflammation Mediators