Proton gradients produced by glucose oxidase microcapsules containing motor F0F1-ATPase for continuous ATP biosynthesis

J Phys Chem B. 2009 Jan 15;113(2):395-9. doi: 10.1021/jp807883e.

Abstract

Glucose oxidase (GOD) microcapsules held together by cross-linker, glutaraldehyde (GA), are fabricated by the layer-by-layer (LbL) assembly technique. The lipid bilayer containing CF(0)F(1)-ATPase was coated on the outer shell of GOD microcapsules. Driven under the proton gradients produced by catalysis of GOD microcapsules for glucose, ATP is synthesized from ADP and inorganic phosphate catalyzed by the ATPase rotary catalysis. The results show here that ATPase reconstituted on the GOD microcapsules retains its catalytic activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / biosynthesis*
  • Aspergillus niger / enzymology
  • Capsules / chemistry
  • Enzyme Stability
  • Glucose Oxidase / chemistry
  • Glucose Oxidase / metabolism*
  • Hydrogen-Ion Concentration
  • Microscopy, Electron, Transmission
  • Proton-Translocating ATPases / metabolism*
  • Protons*

Substances

  • Capsules
  • Protons
  • Adenosine Triphosphate
  • Glucose Oxidase
  • Proton-Translocating ATPases