Abstract
Genetic evidence indicates that plant-specific homologs of DNA-dependent RNA polymerase (Pol) II large subunits form Pol IV and Pol V complexes involved in small interfering RNA production and RNA-directed DNA methylation. Here we describe evidence that Pol V contains subunits shared with Pol II, but that RNA polymerase II subunit (RPB)-4 is missing from Pol V and that RPB5 is present as a Pol V-specific isomer, RPB5b. Pol V also has other proteins that are not present in Pol II, consistent with a role of this complex as an effector of silencing.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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DNA / genetics
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DNA / metabolism
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DNA Polymerase beta / chemistry
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DNA Polymerase beta / genetics
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DNA Polymerase beta / metabolism
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / metabolism
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DNA-Directed RNA Polymerases / chemistry
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DNA-Directed RNA Polymerases / metabolism
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Phenotype
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Protein Subunits / chemistry
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Protein Subunits / metabolism
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RNA Interference*
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RNA Polymerase II / chemistry*
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RNA Polymerase II / metabolism*
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RNA, Small Interfering / genetics
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RNA, Small Interfering / metabolism
Substances
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DNA-Binding Proteins
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Protein Subunits
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RNA, Small Interfering
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DNA
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RNA Polymerase II
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DNA-Directed RNA Polymerases
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DNA Polymerase beta