Nuclear localization of the pre-mRNA associating protein THOC7 depends upon its direct interaction with Fms tyrosine kinase interacting protein (FMIP)

FEBS Lett. 2009 Jan 5;583(1):13-8. doi: 10.1016/j.febslet.2008.11.024. Epub 2008 Dec 4.

Abstract

THOC7 and Fms-interacting protein (FMIP) are members of the THO complex that associate with the mRNA export apparatus. FMIP is a nucleocytoplasmic shuttling protein with a nuclear localization signal (NLS), whereas THOC7 does not contain a typical NLS motif. We show here that THOC7 (50-137, amino acid numbers) binds to the N-terminal portion (1-199) of FMIP directly. FMIP is detected mainly in the nucleus. In the absence of exogenous FMIP, THOC7 resides mainly in the cytoplasm, while in the presence of FMIP, THOC7 is transported into the nucleus with FMIP. Furthermore, THOC7 lacking the FMIP binding site does not co-localize with FMIP, indicating that THOC7/FMIP interaction is required for nuclear localization of THOC7.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Motifs / genetics
  • Animals
  • Cell Nucleus / metabolism*
  • Humans
  • Mice
  • Mutation
  • Nuclear Localization Signals / genetics
  • Nuclear Localization Signals / metabolism
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism*
  • Protein Interaction Mapping
  • RNA Precursors / metabolism*
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism*
  • Xenopus
  • Xenopus Proteins / genetics
  • Xenopus Proteins / metabolism

Substances

  • Nuclear Localization Signals
  • Nuclear Proteins
  • RNA Precursors
  • RNA-Binding Proteins
  • THOC5 protein, human
  • THOC7 protein, human
  • Xenopus Proteins