Gibberellin-induced DELLA recognition by the gibberellin receptor GID1

Nature. 2008 Nov 27;456(7221):459-63. doi: 10.1038/nature07519.

Abstract

Gibberellins control a range of growth and developmental processes in higher plants and have been widely used in the agricultural industry. By binding to a nuclear receptor, GIBBERELLIN INSENSITIVE DWARF1 (GID1), gibberellins regulate gene expression by promoting degradation of the transcriptional regulator DELLA proteins, including GIBBERELLIN INSENSITIVE (GAI). The precise manner in which GID1 discriminates and becomes activated by bioactive gibberellins for specific binding to DELLA proteins remains unclear. Here we present the crystal structure of a ternary complex of Arabidopsis thaliana GID1A, a bioactive gibberellin and the amino-terminal DELLA domain of GAI. In this complex, GID1A occludes gibberellin in a deep binding pocket covered by its N-terminal helical switch region, which in turn interacts with the DELLA domain containing DELLA, VHYNP and LExLE motifs. Our results establish a structural model of a plant hormone receptor that is distinct from the mechanism of the hormone perception and effector recognition of the known auxin receptors.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Motifs
  • Arabidopsis / chemistry*
  • Arabidopsis / metabolism
  • Arabidopsis Proteins / chemistry*
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Circular Dichroism
  • Crystallography, X-Ray
  • Gibberellins / metabolism
  • Gibberellins / pharmacology*
  • Models, Biological
  • Models, Molecular
  • Plant Growth Regulators / metabolism
  • Plant Growth Regulators / pharmacology*
  • Protein Binding
  • Protein Structure, Tertiary / drug effects
  • Receptors, Cell Surface / chemistry*
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / metabolism*
  • Substrate Specificity

Substances

  • Arabidopsis Proteins
  • GAI protein, Arabidopsis
  • GID1a protein, Arabidopsis
  • Gibberellins
  • Plant Growth Regulators
  • Receptors, Cell Surface

Associated data

  • PDB/2ZSH
  • PDB/2ZSI