Abstract
Cyclooxygenase-2 (COX-2) is up-regulated in most high-grade gliomas, and high COX-2 expression is associated with aggressive character and poor prognosis. However, the effect of COX-2 in human glioma cell lines is not well known. This study examined the effect of several stimuli, including interleukin-1beta (IL-1beta) and carcinogens, on COX-2 induction in normal astrocyte cells and human glioma cell lines U87MG, A172, and T98G. IL-1beta-induced COX-2 expression strongly at both protein and messenger ribonucleic acid levels in only the U87MG cells of the glioma cell lines. Furthermore, carcinogen induced COX-2 expression. Similar findings were also observed in normal human astrocyte cells. The U87MG glioma cell line is a good model for COX-2 induction in glioma cell lines.
Publication types
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Comparative Study
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Research Support, N.I.H., Intramural
MeSH terms
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Astrocytes / drug effects*
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Astrocytes / enzymology
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Benzo(a)pyrene / pharmacology
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Carcinogens / pharmacology*
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Cell Line, Tumor / drug effects
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Cell Line, Tumor / enzymology
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Cells, Cultured / drug effects
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Cells, Cultured / enzymology
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Cyclooxygenase 1 / biosynthesis
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Cyclooxygenase 1 / genetics
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Cyclooxygenase 2 / biosynthesis*
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Cyclooxygenase 2 / genetics
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Enzyme Induction / drug effects
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Gene Expression Regulation, Neoplastic / drug effects*
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Glioma / enzymology
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Glioma / genetics
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Glioma / pathology*
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Humans
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Interferon-gamma / pharmacology
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Interleukin-1beta / pharmacology*
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Neoplasm Proteins / biosynthesis*
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Neoplasm Proteins / genetics
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RNA, Messenger / biosynthesis
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RNA, Neoplasm / biosynthesis
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Tetradecanoylphorbol Acetate / pharmacology
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Tumor Necrosis Factor-alpha / pharmacology
Substances
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Carcinogens
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Interleukin-1beta
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Neoplasm Proteins
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RNA, Messenger
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RNA, Neoplasm
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Tumor Necrosis Factor-alpha
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Benzo(a)pyrene
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Interferon-gamma
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Cyclooxygenase 1
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Cyclooxygenase 2
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PTGS1 protein, human
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PTGS2 protein, human
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Tetradecanoylphorbol Acetate