UK epidemic Escherichia coli strains A-E, with CTX-M-15 beta-lactamase, all belong to the international O25:H4-ST131 clone

J Antimicrob Chemother. 2008 Dec;62(6):1241-4. doi: 10.1093/jac/dkn380. Epub 2008 Sep 8.

Abstract

Objectives: Uropathogenic and invasive Escherichia coli O25:H4-ST131 isolates producing CTX-M-15 extended-spectrum beta-lactamase (ESBL) enzymes have recently been shown to be disseminated across the globe. In the UK, many CTX-M-15 ESBL-producing E. coli strains have been previously defined as belonging to the epidemic strains A-E, as determined by PFGE. The present study was carried out to define the relationship between these two groups of pathogenic E. coli.

Methods: Multilocus sequence typing and PFGE were used for molecular characterization of a collection of 61 ESBL-producing E. coli isolates from across the UK.

Results: Strains A to E all belonged to the ST131 clone, further underscoring the epidemiological importance of this lineage.

Conclusions: The future spread of the ST131 clone, and its UK variants, should be monitored closely and the pathogenic mechanisms explaining their success should be investigated.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • Cluster Analysis
  • DNA Fingerprinting
  • Electrophoresis, Gel, Pulsed-Field
  • Escherichia coli / classification*
  • Escherichia coli / enzymology
  • Escherichia coli / isolation & purification*
  • Escherichia coli Infections / epidemiology*
  • Escherichia coli Infections / microbiology*
  • Genotype
  • Humans
  • Molecular Epidemiology
  • Sequence Analysis, DNA
  • Serotyping
  • United Kingdom / epidemiology
  • beta-Lactamases / biosynthesis*

Substances

  • beta-lactamase CTX-M-15
  • beta-Lactamases