Acquisition of granule neuron precursor identity is a critical determinant of progenitor cell competence to form Shh-induced medulloblastoma

Cancer Cell. 2008 Aug 12;14(2):123-34. doi: 10.1016/j.ccr.2008.07.005.

Abstract

Whether the brain tumor medulloblastoma originates from stem cells or restricted progenitor cells is unclear. To investigate this, we activated oncogenic Hedgehog (Hh) signaling in multipotent and lineage-restricted central nervous system (CNS) progenitors. We observed that normal unipotent cerebellar granule neuron precursors (CGNPs) derive from hGFAP(+) and Olig2(+) rhombic lip progenitors. Hh activation in a spectrum of early- and late-stage CNS progenitors generated similar medulloblastomas, but not other brain cancers, indicating that acquisition of CGNP identity is essential for tumorigenesis. We show in human and mouse medulloblastoma that cells expressing the glia-associated markers Gfap and Olig2 are neoplastic and retain features of embryonic-type granule lineage progenitors. Thus, oncogenic Hh signaling promotes medulloblastoma from lineage-restricted granule cell progenitors.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Basic Helix-Loop-Helix Transcription Factors / metabolism
  • Cell Lineage
  • Cerebrum / pathology
  • Crosses, Genetic
  • Female
  • Glial Fibrillary Acidic Protein / metabolism
  • Hedgehog Proteins / metabolism*
  • Humans
  • Male
  • Medulloblastoma / pathology*
  • Mice
  • Nerve Tissue Proteins / metabolism
  • Neurons / pathology*
  • Oligodendrocyte Transcription Factor 2
  • Phenotype
  • Receptors, G-Protein-Coupled
  • Smoothened Receptor
  • Stem Cells / pathology*

Substances

  • Basic Helix-Loop-Helix Transcription Factors
  • Glial Fibrillary Acidic Protein
  • Hedgehog Proteins
  • Nerve Tissue Proteins
  • OLIG2 protein, human
  • Oligodendrocyte Transcription Factor 2
  • Receptors, G-Protein-Coupled
  • SHH protein, human
  • Shh protein, mouse
  • Smo protein, mouse
  • Smoothened Receptor

Associated data

  • GEO/GSE11859