Genotype of human carbonyl reductase CBR3 correlates with doxorubicin disposition and toxicity

Pharmacogenet Genomics. 2008 Jul;18(7):621-31. doi: 10.1097/FPC.0b013e328301a869.

Abstract

Objectives: Doxorubicin is a cytotoxic drug with potential for severe myelosuppression that is highly variable and poorly predictable.

Methods: We correlated CBR1 and CBR3 genotypes with the pharmacokinetics and pharmacodynamics of doxorubicin in 101 Southeast Asian breast cancer patients receiving first-line doxorubicin.

Results: A common CBR3 11G>A variant was associated with lower doxorubicinol area under the concentration-time curve (AUC)/doxorubicin AUC metabolite ratio (P=0.009, GG vs. AA; trend test, P=0.004), lower CBR3 expression in breast tumor tissue (P=0.001, GG vs. AA), greater tumor reduction (P=0.015, GG vs. AA), and greater percentage reduction of leukocyte and platelet counts at nadir (trend test, P < or = 0.03). Chinese and Malays had higher frequency of the CBR3 11G>A variant than Indians (P < or = 0.002). Another variant CBR3 730G>A was associated with higher doxorubicinol AUC (P=0.009, GG vs. AA) and CBR3 expression in breast tumor tissue (P=0.001, GG vs AA).

Conclusion: Polymorphisms in CBR3 may explain interindividual and interethnic variability of doxorubicin pharmacokinetics and pharmacodynamics.

Trial registration: ClinicalTrials.gov NCT00212082.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Alcohol Oxidoreductases / genetics*
  • Alcohol Oxidoreductases / metabolism
  • Antibiotics, Antineoplastic / adverse effects
  • Antibiotics, Antineoplastic / pharmacokinetics
  • Antibiotics, Antineoplastic / therapeutic use
  • Breast Neoplasms / drug therapy*
  • Breast Neoplasms / ethnology
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / metabolism
  • Doxorubicin / adverse effects*
  • Doxorubicin / pharmacokinetics*
  • Doxorubicin / therapeutic use*
  • Ethnicity / genetics
  • Female
  • Genetic Linkage
  • Genotype
  • Humans
  • Inactivation, Metabolic / genetics*
  • Leukocytes / drug effects
  • Leukocytes / pathology
  • Middle Aged
  • Polymorphism, Single Nucleotide

Substances

  • Antibiotics, Antineoplastic
  • Doxorubicin
  • Alcohol Oxidoreductases

Associated data

  • ClinicalTrials.gov/NCT00212082