Eighteen salivary gland pleomorphic adenomas, including nine with a t(3;8)(p21;q12) as the sole chromosome abnormality, were tested for the presence of human papillomavirus (HPV) DNA and for rearrangements of the cellular flanking regions of a HPV 16 integration site mapping to 3p21, as previously described in a cervical carcinoma. In none of the tumors could evidence for either HPV DNA or related sequences, or a rearranged allele of the integration site, be found.