Effects of delays in peripheral blood processing, including cryopreservation, on detection of CD31 expression on naive CD4 T cells

Clin Vaccine Immunol. 2008 Jul;15(7):1141-3. doi: 10.1128/CVI.00430-07. Epub 2008 Apr 30.

Abstract

Delayed processing of peripheral blood or peripheral blood mononuclear cell isolation and cryopreservation can lead to the detection of somewhat higher levels of CD31 expression on naïve CD4 T cells by flow cytometry. These observations should be considered in the planning of multicenter clinical trials and in the interpretation of the results of functional studies.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adult
  • Blood Preservation*
  • Blood Specimen Collection
  • CD4 Lymphocyte Count
  • CD4-Positive T-Lymphocytes / immunology*
  • Cryopreservation
  • HIV Seropositivity / immunology*
  • HIV Seropositivity / virology
  • Humans
  • Middle Aged
  • Platelet Endothelial Cell Adhesion Molecule-1 / immunology
  • Platelet Endothelial Cell Adhesion Molecule-1 / metabolism*
  • Time Factors

Substances

  • Platelet Endothelial Cell Adhesion Molecule-1