Lovastatin biosynthetic genes of Aspergillus terreus are expressed differentially in solid-state and in liquid submerged fermentation

Appl Microbiol Biotechnol. 2008 May;79(2):179-86. doi: 10.1007/s00253-008-1409-2.

Abstract

Molecular studies were performed to establish the causes of the superior lovastatin productivity of a novel solid-state fermentation (SSF) process, in relation with liquid submerged fermentation (SmF; 20 mg/g vs. 0.65 mg/ml). In SSF, biosynthetic genes lovE and lovF transcripts accumulated to high levels from day 1 to day 7. In this period, lovE transcript showed 4.6-fold higher accumulation levels (transcription) than the highest level detected in SmF (day 5). lovF transcript showed two-fold higher expression than the highest point in SmF. In SmF, the expression was only detected clearly on day 5 and, showing a 50% decrease, on day 7. These results show that the higher lovastatin production in SSF is related to a more intense transcription of these biosynthetic genes. A strong expression of gldB gene in lovastatin SSF indicated that Aspergillus terreus senses osmotic stress during the course of SSF, but not in SmF. However, when a liquid medium of identical concentration was used in SmF, lovastatin production decreased in SSF.

MeSH terms

  • Aspergillus / genetics
  • Aspergillus / metabolism*
  • Fermentation*
  • Gene Expression Regulation, Fungal*
  • Genes, Fungal
  • Industrial Microbiology / methods
  • Lovastatin / biosynthesis*
  • Lovastatin / metabolism
  • Transcription, Genetic

Substances

  • Lovastatin