Severe vitamin E deficiency modulates airway allergic inflammatory responses in the murine asthma model

Free Radic Res. 2008 Apr;42(4):387-96. doi: 10.1080/10715760801976600.

Abstract

Allergic asthma is a complex immunologically mediated disease associated with increased oxidative stress and altered antioxidant defenses. It was hypothesized that alpha-tocopherol (alpha-T) decreases oxidative stress and therefore its absence may influence allergic inflammatory process, a pathobiology known to be accompanied by oxidative stress. Therefore, selected parameters of allergic asthma sensitization and inflammation were evaluated following ovalbumin sensitization and re-challenge of alpha-T transfer protein (TTP) knock-out mice (TTP(-/-)) that have greatly reduced lung alpha-T levels (e.g.<5%) compared to their litter mate controls (TTP(+/+)). Results showed that severe alpha-T deficiency result in a blunted lung expression of IL-5 mRNA and IL-5 protein and plasma IgE levels compared with TTP(+/+) mice following immune sensitization and rechallenge, although lung lavage eosinophil levels were comparable in both genomic strains. It is concluded that the initial stimulation of immune responses by the TTP(-/-) mice were generally blunted compared to the TTP(+/+) mice, thus diminishing some aspects of subsequent allergic inflammatory processes.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Asthma / genetics
  • Asthma / metabolism*
  • Asthma / pathology*
  • Bronchial Hyperreactivity / metabolism
  • Carrier Proteins / genetics
  • Eosinophils / metabolism
  • Gene Expression Regulation*
  • Immunoglobulin E / blood
  • Inflammation
  • Interleukin-5 / metabolism
  • Lung / pathology
  • Mice
  • Mice, Knockout
  • Ovalbumin / chemistry
  • Oxidative Stress
  • Vitamin E Deficiency / complications*

Substances

  • Carrier Proteins
  • Interleukin-5
  • alpha-tocopherol transfer protein
  • Immunoglobulin E
  • Ovalbumin