Cytokine levels in sputum of patients with tracheostomy and profound multiple disabilities

Cytokine. 2008 Apr;42(1):71-6. doi: 10.1016/j.cyto.2008.01.012. Epub 2008 Mar 7.

Abstract

Background: Airway immunopathogenesis is unclear in patients with profound multiple disabilities (PMD) who undergo tracheostomy.

Methods: The levels of tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), IL-6, IL-8, IL-10, and IL-12p70 cytokines were determined in sputum of 28 patients with PMD who underwent tracheostomy and in 14 healthy subjects, using a cytometric bead array.

Results: The concentrations of IL-1beta, IL-6 and IL-8 in the patients were significantly higher than those in controls (p < 0.001). IL-6, and IL-8 levels in eight PMD patients in the febrile period were significantly higher than those in the afebrile period (p < 0.01 and p < 0.05, respectively). Serum CRP levels were slightly elevated in 12 of the 28 patients (43%) in the afebrile period, but there were no significant differences in the level of any cytokine between patients with normal and elevated serum CRP.

Conclusion: PMD patients with tracheostomy have chronic airway inflammation.

MeSH terms

  • Adolescent
  • Adult
  • C-Reactive Protein / metabolism
  • Child
  • Child, Preschool
  • Cytokines / metabolism*
  • Disabled Persons*
  • Female
  • Humans
  • Inflammation*
  • Male
  • Microarray Analysis
  • Middle Aged
  • Sputum / immunology*
  • Trachea / immunology
  • Trachea / pathology
  • Trachea / surgery
  • Tracheostomy*

Substances

  • Cytokines
  • C-Reactive Protein