Molecular cloning of cDNA encoding the 16 KDa subunit of vacuolar H(+)-ATPase from mouse cerebellum

Biochem Biophys Res Commun. 1991 May 15;176(3):1062-7. doi: 10.1016/0006-291x(91)90391-j.

Abstract

cDNA for the 16 kDa subunit of vacuolar H(+)-ATPase was cloned from mouse cerebellum and sequenced. The deduced polypeptide (155 amino acid residues; molecular weight, 15,808) was highly hydrophobic and homologous to the subunits of bovine adrenal medulla, Torpedo marmorata electric lobe, Drosophila and yeast. Glu-139 (supposed to be essential for proton transport) was also conserved as the potential dicyclohexylcarbodiimide binding site. The subunit had four transmembrane segments: Segment II and IV were highly homologous and Glu-139 was located in Segment IV. The roles of the non-conserved regions are discussed.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenal Medulla / enzymology
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • Cattle
  • Cerebellum / enzymology*
  • Cloning, Molecular
  • DNA / genetics*
  • Drosophila / enzymology
  • Electric Organ / enzymology
  • Escherichia coli / genetics
  • Gene Library
  • Macromolecular Substances
  • Mice
  • Mice, Inbred ICR
  • Molecular Sequence Data
  • Molecular Weight
  • Oligonucleotide Probes
  • Proton-Translocating ATPases / genetics*
  • Restriction Mapping
  • Saccharomyces cerevisiae / enzymology
  • Sequence Homology, Nucleic Acid
  • Torpedo
  • Vacuoles / enzymology*

Substances

  • Macromolecular Substances
  • Oligonucleotide Probes
  • DNA
  • Proton-Translocating ATPases