Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster

Nat Methods. 2008 Jan;5(1):49-51. doi: 10.1038/nmeth1146. Epub 2007 Dec 16.

Abstract

The conditional expression of hairpin constructs in Drosophila melanogaster has emerged in recent years as a method of choice in functional genomic studies. To date, upstream activating site-driven RNA interference constructs have been inserted into the genome randomly using P-element-mediated transformation, which can result in false negatives due to variable expression. To avoid this problem, we have developed a transgenic RNA interference vector based on the phiC31 site-specific integration method.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Drosophila Proteins / metabolism*
  • Drosophila melanogaster / genetics*
  • Gene Targeting / methods*
  • Genetic Vectors / genetics*
  • RNA Interference*

Substances

  • Drosophila Proteins