Protective effect of irisolidone, a metabolite of kakkalide, against hydrogen peroxide induced cell damage via antioxidant effect

Bioorg Med Chem. 2008 Feb 1;16(3):1133-41. doi: 10.1016/j.bmc.2007.10.085. Epub 2007 Oct 30.

Abstract

The protective properties of irisolidone (a metabolite of kakkalide by intestinal bacteria) against hydrogen peroxide (H(2)O(2)) induced cell damage were investigated. Irisolidone was found to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, and the intracellular reactive oxygen species (ROS), thereby preventing lipid peroxidation and DNA damage. Irisolidone inhibited apoptosis in Chinese hamster lung fibroblast (V79-4) cells induced by H(2)O(2) via radical scavenging activity. This was achieved by the activation of the extracellular signal regulated kinase (ERK) and DNA binding activity of activator protein-1 (AP-1) (a downstream transcription factor of ERK) by irisolidone.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / metabolism*
  • Apoptosis / drug effects*
  • Cell Line
  • Cricetinae
  • Cytoprotection / drug effects
  • DNA Damage / genetics
  • Enzyme Activation / drug effects
  • Extracellular Signal-Regulated MAP Kinases / metabolism
  • Flavonoids / chemistry
  • Flavonoids / pharmacology*
  • Free Radicals / metabolism
  • Glycosides / chemistry
  • Glycosides / metabolism*
  • Humans
  • Hydrogen Peroxide / pharmacology*
  • Intestinal Mucosa / metabolism
  • Intestines / microbiology
  • Isoflavones / chemistry
  • Isoflavones / metabolism*
  • Lipid Peroxidation / drug effects
  • Molecular Structure
  • Transcription Factor AP-1 / metabolism

Substances

  • Antioxidants
  • Flavonoids
  • Free Radicals
  • Glycosides
  • Isoflavones
  • Transcription Factor AP-1
  • irisolidone
  • kakkalide
  • Hydrogen Peroxide
  • Extracellular Signal-Regulated MAP Kinases