A highly sensitive and specific multiplex RT-PCR to detect foot-and-mouth disease virus in tissue and food samples

H-F Bao; D Li; J-H Guo; Z-J Lu; Y-L Chen; Z-X Liu; X-T Liu; Q-G Xie

doi:10.1007/s00705-007-1082-y

A highly sensitive and specific multiplex RT-PCR to detect foot-and-mouth disease virus in tissue and food samples

Arch Virol. 2008;153(1):205-9. doi: 10.1007/s00705-007-1082-y. Epub 2007 Nov 8.

Authors

H-F Bao¹, D Li, J-H Guo, Z-J Lu, Y-L Chen, Z-X Liu, X-T Liu, Q-G Xie

Affiliation

¹ Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiologic Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China.

PMID: 17987350
DOI: 10.1007/s00705-007-1082-y

Abstract

Three sets of primers to detect foot-and-mouth disease virus (FMDV) using multiplex RT-PCR were designed based on several reference nucleotide sequences, and their reaction conditions were determined. By testing ten-fold serial dilutions of FMDV, the sensitivity of multiplex RT-PCR is 100 times higher than conventional RT-PCR. Meanwhile, its specificity was confirmed compared with other related vesicular disease viruses. Furthermore, 30 field samples from different animals were tested, and the results supported the method's potential applications in routine veterinary quarantine and epidemic surveillance of FMDV.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle
Food Microbiology*
Foot-and-Mouth Disease / diagnosis*
Foot-and-Mouth Disease Virus / genetics
Foot-and-Mouth Disease Virus / isolation & purification*
RNA, Viral / analysis*
Reverse Transcriptase Polymerase Chain Reaction / methods*
Sensitivity and Specificity

Substances

RNA, Viral