Dissecting timing variability in yeast meiosis

Cell. 2007 Nov 2;131(3):544-56. doi: 10.1016/j.cell.2007.09.044.

Abstract

Cell-to-cell variability in the timing of cell-fate changes can be advantageous for a population of single-celled organisms growing in a fluctuating environment. We study timing variability during meiosis in Saccharomyces cerevisiae, initiated upon nutritional starvation. We use time-lapse fluorescence microscopy to measure the timing of meiotic events in single cells and find that the duration of meiosis is highly variable between cells. This variability is concentrated between the beginning of starvation and the onset of early meiosis genes. Cell-cycle variability and nutritional history have little effect on this timing variability. Rather, variation in the production rate of the meiotic master regulator Ime1 and its gradual increase over time govern this variability, and cell size effects are channeled through Ime1. These results tie phenotypic variability with expression dynamics of a transcriptional regulator and provide a general framework for the study of temporal developmental processes.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Cycle Proteins / metabolism
  • Cell Nucleus / metabolism
  • DNA-Binding Proteins / metabolism
  • Food
  • Genes, Fungal
  • Genes, Reporter
  • Meiosis*
  • Nuclear Proteins / metabolism
  • Phenotype
  • Saccharomyces cerevisiae / cytology*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae Proteins / metabolism
  • Signal Transduction
  • Time Factors
  • Transcription Factors / metabolism

Substances

  • Cell Cycle Proteins
  • DMC1 protein, S cerevisiae
  • DNA-Binding Proteins
  • IME1 protein, S cerevisiae
  • Nuclear Proteins
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors