Engineered cardiac tissues for in vitro assessment of contractile function and repair mechanisms

Do Eun Kim; Eun Jung Lee; Timothy P Martens; Rina Kara; Hina W Chaudhry; Silviu Itescu; Kevin D Costa

doi:10.1109/IEMBS.2006.259753

Engineered cardiac tissues for in vitro assessment of contractile function and repair mechanisms

Conf Proc IEEE Eng Med Biol Soc. 2006:2006:849-52. doi: 10.1109/IEMBS.2006.259753.

Authors

Do Eun Kim¹, Eun Jung Lee, Timothy P Martens, Rina Kara, Hina W Chaudhry, Silviu Itescu, Kevin D Costa

Affiliation

¹ Department of Biomedical Engineering, Columbia University, 1210 Amsterdam Avenue, New York, NY 10027, USA. dk2194@columbia.edu

PMID: 17946863
DOI: 10.1109/IEMBS.2006.259753

Abstract

For efficiently assessing the potential for grafted cells to repair infarcted myocardium, a simplified surrogate heart muscle system would offer numerous advantages. Using neonatal rat cardiac myocytes in a collagen matrix, we created thin cylindrical engineered cardiac tissues (ECTs) that exhibit essential aspects of physiologic cardiac muscle function. Furthermore, a novel cryo-injured ECT model of myocardial infarction offers the potential for the longitudinal study of mechanisms of cell-based cardiac repair in vitro.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Animals, Newborn
Cell Culture Techniques / methods
Cells, Cultured
Heart / physiology*
Myocardial Contraction / physiology*
Myocytes, Cardiac / cytology
Myocytes, Cardiac / physiology*
Rats
Rats, Sprague-Dawley
Regeneration / physiology*
Tissue Engineering / methods*