Gender-specific effect of overexpression of sFlt-1 in pregnant mice on fetal programming of blood pressure in the offspring later in life

Am J Obstet Gynecol. 2007 Oct;197(4):418.e1-5. doi: 10.1016/j.ajog.2007.06.064.

Abstract

Objective: The purpose of this study was to determine whether fetal programming of adult blood pressure is altered in a previously characterized mouse model of preeclampsia that was induced by sFlt-1.

Study design: CD-1 mouse mothers at day 8 of gestation were injected with an adenovirus carrying Flt 1-3 (10(9) plaque-forming units) or with an adenovirus carrying mFc as control (10(9) plaque-forming units). The resulting pups were followed until 6 months of age, at which time blood pressure (BP) was recorded continuously for 6 days. The offspring weight was also recorded from weaning until adulthood.

Results: BP was significantly higher in the male offspring that were born to sFlt-1-treated mothers compared with the controls. Male offspring from sFlt-1-treated mothers were significantly smaller from weaning until adulthood. However, there were no significant differences in BP and postweaning weight in female offspring between the 2 groups.

Conclusion: Our findings highlight the role of the intrauterine environment in the developmental origin of adult disease.

MeSH terms

  • Animals
  • Animals, Newborn
  • Birth Weight / genetics
  • Birth Weight / physiology
  • Blood Pressure / physiology*
  • Female
  • Fetal Development / physiology*
  • Male
  • Mice
  • Pregnancy
  • Random Allocation
  • Sex Factors
  • Telemetry
  • Transfection
  • Vascular Endothelial Growth Factor Receptor-1 / biosynthesis*
  • Vascular Endothelial Growth Factor Receptor-1 / genetics

Substances

  • Flt1 protein, mouse
  • Vascular Endothelial Growth Factor Receptor-1