Abstract
Two isocoumarin derivatives, stoloniferol A (1) and B (2), a known 5alpha, 8alpha-epidioxy-23-methyl-(22E, 24R)-ergosta-6, 22-dien-3beta-ol (3), and a known dihydrocitrinone (4) were isolated from the ethyl acetate extract of the sea squirt-derived fungus, Penicillium stoloniferum QY2-10, and a halophilic fungus, Penicillium notatum B-52, respectively. Their structures were elucidated by spectroscopic methods and optical rotation. The stereochemistry of 2 was determined on the basis of different NOE experiments and chemical transformation. Compound 3 showed cytotoxicity against P388 cells, with an IC50 value of 4.07 microM.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antineoplastic Agents / chemistry
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Antineoplastic Agents / isolation & purification*
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Antineoplastic Agents / pharmacology
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Cell Line, Tumor
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Cell Survival / drug effects
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Chromatography, High Pressure Liquid
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Humans
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Inhibitory Concentration 50
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Isocoumarins / chemistry
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Isocoumarins / isolation & purification*
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Isocoumarins / pharmacology
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Molecular Structure
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Nuclear Magnetic Resonance, Biomolecular
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Penicillium / chemistry
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Penicillium / isolation & purification
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Penicillium chrysogenum / chemistry*
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Penicillium chrysogenum / isolation & purification
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Seawater
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Urochordata / microbiology*
Substances
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Antineoplastic Agents
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Isocoumarins