Abstract
In aplastic anaemia, T cells have a central role in the pathophysiology of bone marrow destruction. This study showed that T cells from patients with aplastic anaemia expressed decreased T-cell receptor (TCR) zeta-chain protein and mRNA levels compared to healthy controls. Patients with decreased TCR zeta-chain showed an abnormal response in intracellular calcium following stimulation through the TCR. We also observed an altered pattern of the transcription factors CREMalpha and Elf-1 that are implicated in zeta-chain transcription. We concluded that TCR zeta-chain expression was decreased in the majority of patients with aplastic anaemia, regardless of disease activity or treatment status.
Publication types
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Research Support, N.I.H., Intramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Adolescent
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Adult
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Aged
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Anemia, Aplastic / immunology*
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Calcium / metabolism
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Case-Control Studies
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Child
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Cyclic AMP Response Element Modulator / metabolism
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Ephrin-A2 / metabolism
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Female
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Gene Expression Regulation, Neoplastic*
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Humans
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Intracellular Fluid / metabolism
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Male
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Membrane Proteins / genetics*
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Middle Aged
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Polymerase Chain Reaction
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Promoter Regions, Genetic / genetics*
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RNA, Messenger / analysis*
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Receptors, Antigen, T-Cell / genetics*
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Sequence Analysis, DNA
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T-Lymphocytes / metabolism*
Substances
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Ephrin-A2
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Membrane Proteins
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RNA, Messenger
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Receptors, Antigen, T-Cell
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antigen T cell receptor, zeta chain
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Cyclic AMP Response Element Modulator
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Calcium