Sperm immobilized before intracytoplasmic sperm injection undergo ultrastructural damage and acrosomal disruption

Maria José Gómez-Torres; Jorge Ten; José L Girela; Jesús Romero; Rafael Bernabeu; Joaquin De Juan

doi:10.1016/j.fertnstert.2006.12.063

Sperm immobilized before intracytoplasmic sperm injection undergo ultrastructural damage and acrosomal disruption

Fertil Steril. 2007 Sep;88(3):702-4. doi: 10.1016/j.fertnstert.2006.12.063. Epub 2007 Jun 4.

Authors

Maria José Gómez-Torres¹, Jorge Ten, José L Girela, Jesús Romero, Rafael Bernabeu, Joaquin De Juan

Affiliation

¹ Department of Biotechnology, University of Alicante, Alicante, Spain. mjose.gomez@ua.es <mjose.gomez@ua.es>

PMID: 17548082
DOI: 10.1016/j.fertnstert.2006.12.063

Abstract

The aim of this study was to describe morphologic sperm changes observed via scanning electron microscopy and the acrosomal status resulting from immobilization and micromanipulation. The manipulations made on sperm before intracytoplasmic sperm injection were the possible cause of sperm ultrastructural damages and could trigger an acrosome reaction.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acrosome / pathology*
Acrosome / ultrastructure
Female
Humans
Image Processing, Computer-Assisted
Male
Microscopy, Electron, Scanning
Pregnancy
Sperm Injections, Intracytoplasmic / methods*
Sperm Motility / physiology*
Spermatozoa / pathology*
Spermatozoa / ultrastructure