Abstract
Working with the antimetabolic chemopredictivity assay on short term cultures we evidenced a correlation between in vitro chemosensitivity of colorectal cancer and fractional incorporation of radionucleotides. Four different drugs (5-FU, mitomycin C, 4'-iododeoxydoxorubicin and ruboxyl, a nitroxyl derivative of daunorubicin) were tested on 102 tumor cultures. The extent of 3H-TdR and 3H-UdR incorporation into DNA and RNA in the related control cultures was in relationship with the chemosensitivity of the tumor. Along with the labeling index this simple metabolic-kinetic trait may gain a role for the screening of tumor phenotypes sensitive to chemotherapy.
MeSH terms
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Antineoplastic Agents / pharmacology*
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Colorectal Neoplasms / drug therapy*
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Colorectal Neoplasms / metabolism
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DNA, Neoplasm / biosynthesis
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Daunorubicin / analogs & derivatives
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Daunorubicin / pharmacology
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Doxorubicin / analogs & derivatives
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Doxorubicin / pharmacology
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Humans
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In Vitro Techniques
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Liver Neoplasms / secondary
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Mitomycin / pharmacology
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RNA, Neoplasm / biosynthesis
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Thymidine / metabolism*
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Tritium
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Tumor Cells, Cultured / drug effects
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Tumor Cells, Cultured / metabolism
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Uridine / metabolism*
Substances
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Antineoplastic Agents
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DNA, Neoplasm
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RNA, Neoplasm
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Tritium
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Mitomycin
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4'-deoxy-4'-iododoxorubicin
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Doxorubicin
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ruboxyl
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Thymidine
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Uridine
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Daunorubicin