[A pseudo-outbreak of pharyngeal gonorrhoea related to a false-positive PCR-result]

Ned Tijdschr Geneeskd. 2007 Mar 24;151(12):689-91.
[Article in Dutch]

Abstract

Nucleic acid amplification tests, including the polymerase chain reaction (PCR), are sensitive and specific tests that are often used for diagnosing sexually transmitted diseases (STDs). A pseudo-outbreak of pharyngeal gonorrhoea in a group of prostitutes turned out to have been caused by false-positive test results due to commensal oropharyngeal Neisseria species. Specific molecular tests may yield erroneous results. When the results of an STD study have major consequences at a legal or social level, it is advisable, in consultation with a medical microbiologist, to take a sample for culture or to carry out a second molecular test aimed at a different part of the bacterial genome.

Publication types

  • Review

MeSH terms

  • Diagnosis, Differential
  • Disease Outbreaks
  • False Positive Reactions
  • Female
  • Gonorrhea / diagnosis*
  • Gonorrhea / epidemiology
  • Humans
  • Neisseria gonorrhoeae / isolation & purification*
  • Nucleic Acid Amplification Techniques
  • Pharyngeal Diseases / diagnosis*
  • Pharyngeal Diseases / epidemiology
  • Polymerase Chain Reaction / methods*
  • Polymerase Chain Reaction / standards*
  • Sensitivity and Specificity
  • Sex Work