Objectives: To develop a kinetic, inexpensive, automatable assay for ceruloplasmin as ferroxidase.
Design and methods: Dithiothreitol has been used to stabilize the substrate and quinolones to complex the ferric.
Results: Mean ferroxidase activity in healthy adults, Wilson's disease and pregnancy was 787.29, 178.5 and 1828.09 IU/L, respectively. Correlation coefficient of ferroxidase vis-à-vis copper, ferroxidase by ferrozine and ceruloplasmin were 0.90, 0.94 and 0.92, respectively.
Conclusions: Norfloxacin method is a simple, inexpensive and automatable kinetic assay.