Induction of members of the IL-8/NAP-1 gene family in human T lymphocytes is suppressed by cyclosporin A

Biochem Biophys Res Commun. 1991 Nov 27;181(1):179-83. doi: 10.1016/s0006-291x(05)81398-1.

Abstract

Human members of a family of structurally related cytokines, which play a role as effectors of inflammation, were analyzed for their expression and regulation in T lymphocytes. Members of this gene family include Platelet Basic Protein (PBP); Platelet Factor 4 (PF-4); IL-8/NAP-1; IP-10, a gamma interferon induced protein; GRO; pAT 464 and pAT 744. In resting T lymphocytes the RNAs of the individual genes could not be detected, but all genes were induced upon stimulation with PHA or with PHA/PMA. The induction of five genes was blocked by the immunosuppresive drug cyclosporin A (CSA), which appears to affect initial events in T cell activation. This expression in T lymphocytes, especially the sensitivity to CSA, indicates a common immunmodulatory role of these structural related proteins.

MeSH terms

  • Blotting, Northern
  • Cell Line
  • Cells, Cultured
  • Cyclosporine / pharmacology*
  • DNA Probes
  • Gene Expression Regulation / drug effects
  • Humans
  • Interleukin-8 / genetics*
  • Kinetics
  • Lymphocyte Activation
  • Multigene Family* / drug effects
  • RNA / genetics
  • RNA / isolation & purification
  • RNA, Messenger / genetics
  • T-Lymphocytes / drug effects
  • T-Lymphocytes / immunology*
  • Transcription, Genetic / drug effects

Substances

  • DNA Probes
  • Interleukin-8
  • RNA, Messenger
  • RNA
  • Cyclosporine