Flow cytometric reticulocyte analysis using thiazole orange; clinical experience and technical limitations

I Chin-Yee; M Keeney; R C Lohmann

doi:10.1111/j.1365-2257.1991.tb00267.x

Flow cytometric reticulocyte analysis using thiazole orange; clinical experience and technical limitations

Clin Lab Haematol. 1991;13(2):177-88. doi: 10.1111/j.1365-2257.1991.tb00267.x.

Authors

I Chin-Yee¹, M Keeney, R C Lohmann

Affiliation

¹ University of Western Ontario, Department of Hematology/Blood Bank, Victoria Hospital Corporation, London, Canada.

PMID: 1718653
DOI: 10.1111/j.1365-2257.1991.tb00267.x

Abstract

Flow cytometric (FCM) reticulocyte analysis using thiazole orange (TO) is becoming an increasingly popular method for routine quantification of reticulocytes. The methodology is accurate, cost-effective and shows a high correlation with manual techniques. We describe our experience with the clinical application of FCM reticulocyte analysis in a general hospital setting over a 20-month period with special emphasis on technical limitations.

MeSH terms

Antineoplastic Agents / therapeutic use
Benzothiazoles
Bone Marrow Transplantation / pathology
Combined Modality Therapy
Erythrocyte Count / methods
Female
Flow Cytometry
Fluorescent Dyes*
Humans
Male
Quinolines
RNA / blood*
Reference Values
Reproducibility of Results
Reticulocytes / cytology*
Thiazoles*

Substances

Antineoplastic Agents
Benzothiazoles
Fluorescent Dyes
Quinolines
Thiazoles
thiazole orange
RNA