SCN9A mutations in paroxysmal extreme pain disorder: allelic variants underlie distinct channel defects and phenotypes

Neuron. 2006 Dec 7;52(5):767-74. doi: 10.1016/j.neuron.2006.10.006.

Abstract

Paroxysmal extreme pain disorder (PEPD), previously known as familial rectal pain (FRP, or OMIM 167400), is an inherited condition characterized by paroxysms of rectal, ocular, or submandibular pain with flushing. A genome-wide linkage search followed by mutational analysis of the candidate gene SCN9A, which encodes hNa(v)1.7, identified eight missense mutations in 11 families and 2 sporadic cases. Functional analysis in vitro of three of these mutant Na(v)1.7 channels revealed a reduction in fast inactivation, leading to persistent sodium current. Other mutations in SCN9A associated with more negative activation thresholds are known to cause primary erythermalgia (PE). Carbamazepine, a drug that is effective in PEPD, but not PE, showed selective block of persistent current associated with PEPD mutants, but did not affect the negative activation threshold of a PE mutant. PEPD and PE are allelic variants with distinct underlying biophysical mechanisms and represent a separate class of peripheral neuronal sodium channelopathy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Amino Acid Sequence
  • Analgesics, Non-Narcotic / pharmacology
  • Carbamazepine / pharmacology
  • Cell Line
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA Mutational Analysis
  • Electrophysiology
  • Genetic Linkage / physiology
  • Genetic Variation
  • Genotype
  • Humans
  • Molecular Sequence Data
  • Mutation / physiology*
  • NAV1.7 Voltage-Gated Sodium Channel
  • Neuralgia / genetics*
  • Neuralgia / physiopathology
  • Patch-Clamp Techniques
  • Pedigree
  • Phenotype
  • Sodium Channel Blockers
  • Sodium Channels / drug effects
  • Sodium Channels / genetics*
  • Sodium Channels / physiology*
  • Transfection

Substances

  • Analgesics, Non-Narcotic
  • NAV1.7 Voltage-Gated Sodium Channel
  • SCN9A protein, human
  • Sodium Channel Blockers
  • Sodium Channels
  • Carbamazepine