Developmental localization of potassium chloride co-transporter 2 (KCC2) in the Purkinje cells of embryonic mouse cerebellum

Chitoshi Takayama; Yoshiro Inoue

doi:10.1016/j.neures.2006.10.016

Developmental localization of potassium chloride co-transporter 2 (KCC2) in the Purkinje cells of embryonic mouse cerebellum

Neurosci Res. 2007 Feb;57(2):322-5. doi: 10.1016/j.neures.2006.10.016. Epub 2006 Nov 28.

Authors

Chitoshi Takayama¹, Yoshiro Inoue

Affiliation

¹ Department of Molecular Neuroanatomy, Hokkaido University School of Medicine, Kita-15 Nishi-7, Sapporo, Japan. takachan@med.hokudai.ac.jp

PMID: 17134779
DOI: 10.1016/j.neures.2006.10.016

Abstract

Developmental shift in GABA actions from depolarization to hyperpolarization occurs as a result of decreasing the intracellular Cl(-) concentration regulated by K(+)-Cl(-) co-transporter 2 (KCC2). To clarify the time-course of the developmental shift on the Purkinje cells, we examined KCC2-localization in the embryonic mouse cerebellum. The KCC2 was first detected within the Purkinje cells in the Purkinje cell layer of the hemisphere at embryonic day 15 (E15) and the vermis at E17, but the ventricular and intermediate zones were negative. These results suggest that GABA might become inhibitory on the Purkinje cells after their settling in the Purkinje cell layer.

MeSH terms

Age Factors
Animals
Cerebellum / cytology*
Embryo, Mammalian
Gene Expression Regulation, Developmental / physiology*
K Cl- Cotransporters
Mice
Mice, Inbred C57BL
Microscopy, Immunoelectron / methods
Purkinje Cells / metabolism*
Purkinje Cells / ultrastructure
Symporters / metabolism*
Symporters / ultrastructure

Substances

Symporters