Physiology of electrogenic SLC26 paralogues

Novartis Found Symp. 2006:273:126-38; discussion 138-47, 261-4.

Abstract

SLC26 anion exchangers transport monovalent and divalent anions, with a diversity of anion specificity and stoichiometry. Our microelectrode studies indicate that several SLC26 members are electrogenic. We reported that Slc26a6 functions as a Cl-/formate, Cl-/oxalate, Cl-/OH- and electrogenic Cl-/nHCO3- exchanger. Recently, we have also confirmed that Slc26a7 does not behave as a Cl-/HCO3- exchanger but does function as an electrogenic anion conductance, perhaps a channel. We have also cloned murine Slc26a9, which is strongly expressed in the respiratory tract and stomach. Radioisotope uptakes in Xenopus oocytes indicate that Slc26a9 is a highly selective anion exchanger, transporting Cl- but neither formate, oxalate, nor SO42-. We also utilized electrophysiology to voltage clamp (VC) and/or measure intracellular pH (pHi), Cl- ([Cl-],) and Na+ ([Na+]i), in response to various ion replacements. Cl- removal in HCO3- depolarizes oocytes (to > +60mV), alkalinizes oocytes, and decreases aCl-i. Slc26a9 thus functions as an electrogenic nCl-/HCO3- exchanger, suggesting a role in pulmonary and gastric HCO3- secretion and/or CO2 transport. VC experiments revealed channel-like currents (>10 microA at -60mV and >80 microA at +60mV) mediated by Slc26a9 in the presence and absence of HCO3-. Our experiments and those of others continue to reveal additional characteristics and unique roles for this new class of electrogenic anion transporters.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Antiporters / genetics
  • Antiporters / metabolism*
  • Chlorides / metabolism
  • Cloning, Molecular
  • Ion Transport
  • Mice
  • Models, Biological
  • Oocytes
  • Patch-Clamp Techniques
  • Protein Transport
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Sequence Homology*
  • Stomach / cytology
  • Sulfate Transporters

Substances

  • Antiporters
  • Chlorides
  • RNA, Messenger
  • SLC26A9 protein, human
  • Sulfate Transporters