Association of neuropeptides with Th1/Th2 balance and allergic sensitization in children

Clin Exp Allergy. 2006 Nov;36(11):1408-16. doi: 10.1111/j.1365-2222.2006.02576.x.

Abstract

Background: Among neurogenic factors, the neuropeptides have an important regulatory influence on immune system activity and may lead to allergic sensitization.

Objective: The aim of our study was to investigate the relationship of the neuropeptides vasoactive intestinal peptide (VIP), somatostatin (SOM) and substance P (SP) on modulation of Th1/Th2 balance and allergic sensitization in children.

Methods: Within the LISAplus (Life style-Immune system-Allergy) study, blood samples of 321 six-year-old children were analysed for concentration of neuropeptides, Th1 and Th2 cytokines, transcription factors for T cell regulation and suppressors of cytokine signalling. In addition, samples were screened for specific IgE against inhalant and food allergens.

Results: Children with high SOM values showed a Th2 polarization and a reduced expression of FOXP3, the marker for regulatory T cells. High (VIP) levels correlated inversely with the expression of T cell transcription factors (Tbet and SOCS3). In contrast, elevated levels of SP were associated with reduced GATA3 and SOCS3 expression and with increased IFN-gamma concentrations. Allergic sensitization was more prevalent in children with higher SOM and VIP concentrations but not associated with SP levels.

Conclusion: Our data reveal an association between neuropeptides and modulatory effects on immune cells in vivo, especially on Th1/Th2 balance with a correlation to allergic sensitization in children. We suggest that elevated SOM and VIP concentrations and the inducing factors should be considered as allergy risk factors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers / blood
  • Child
  • Female
  • Food Hypersensitivity / immunology
  • Forkhead Transcription Factors / genetics
  • GATA3 Transcription Factor / genetics
  • Humans
  • Hypersensitivity / blood*
  • Immunoglobulin E / blood
  • Interferon-gamma / blood
  • Interleukin-4 / blood
  • Interleukin-4 / genetics
  • Interleukin-5 / blood
  • Interleukin-9 / blood
  • Logistic Models
  • Male
  • Neuropeptides / blood*
  • RNA, Messenger / analysis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Somatostatin / blood
  • Substance P / blood
  • Suppressor of Cytokine Signaling 1 Protein
  • Suppressor of Cytokine Signaling 3 Protein
  • Suppressor of Cytokine Signaling Proteins / genetics
  • Th1 Cells / immunology*
  • Th2 Cells / immunology*
  • Vasoactive Intestinal Peptide / blood

Substances

  • Biomarkers
  • FOXP3 protein, human
  • Forkhead Transcription Factors
  • GATA3 Transcription Factor
  • GATA3 protein, human
  • Interleukin-5
  • Interleukin-9
  • Neuropeptides
  • RNA, Messenger
  • SOCS1 protein, human
  • SOCS3 protein, human
  • Suppressor of Cytokine Signaling 1 Protein
  • Suppressor of Cytokine Signaling 3 Protein
  • Suppressor of Cytokine Signaling Proteins
  • Interleukin-4
  • Substance P
  • Vasoactive Intestinal Peptide
  • Immunoglobulin E
  • Somatostatin
  • Interferon-gamma