Abstract
The transcriptional factor CaTup1p represses many genes involved in intracellular processes, including the yeast-hypha transition, in the human fungal pathogen Candida albicans. Using tandem affinity purification technology, we identified a novel protein that interacts with CaTup1p, named Tcc1p (Tup1p complex component). Tcc1p is a C. albicans-specific protein with a 736-amino-acid polypeptide with four tetratricopeptide repeat (TPR) motifs in the N-terminal portion. Tcc1p formed a protein complex with CaTup1p via the TPR domain of Tcc1p, independently of CaSsn6p-CaTup1p The tcc1Delta disruptant showed filamentous growth under conditions inducing the yeast form, as is true of the Catup1Delta mutant. Consistent with this result, the common set of hypha-specific genes was negatively regulated by both TCC1 and CaTUP1. These observations will provide new insights into CaTup1p-dependent transcriptional gene regulation in C. albicans.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Candida albicans / cytology
-
Candida albicans / genetics
-
Candida albicans / pathogenicity*
-
Candida albicans / physiology*
-
DNA-Binding Proteins / genetics
-
DNA-Binding Proteins / metabolism
-
Fungal Proteins / genetics
-
Fungal Proteins / metabolism*
-
Gene Expression Regulation, Fungal
-
Humans
-
Hyphae / physiology
-
Mice
-
Molecular Sequence Data
-
Multiprotein Complexes / metabolism
-
Nuclear Proteins / genetics
-
Nuclear Proteins / metabolism
-
Phenotype
-
Repressor Proteins / genetics
-
Repressor Proteins / metabolism
-
Saccharomyces cerevisiae Proteins / genetics
-
Saccharomyces cerevisiae Proteins / metabolism
-
Sequence Alignment
-
Transcription, Genetic
-
Two-Hybrid System Techniques
Substances
-
CYC8 protein, S cerevisiae
-
DNA-Binding Proteins
-
Fungal Proteins
-
Multiprotein Complexes
-
Nuclear Proteins
-
Repressor Proteins
-
Saccharomyces cerevisiae Proteins
-
TUP1 protein, S cerevisiae