Decreased expression of thyroid receptor-associated protein 220 in temporal lobe tissue of patients with refractory epilepsy

Biochem Biophys Res Commun. 2006 Oct 6;348(4):1389-97. doi: 10.1016/j.bbrc.2006.08.010. Epub 2006 Aug 10.

Abstract

Purpose: TRAP220 (thyroid hormone receptor-associated protein) functions as a coactivator for nuclear receptors and stimulates transcription by recruiting the TRAP mediator complex to hormone responsive promoter regions. Thus, TRAP220 enhances the function of thyroid/steroid hormone receptors such as thyroid hormone and oestrogen receptors. This study investigated the expression of TRAP220 mRNA and protein level in epileptic brains comparing with human control.

Methods: We examined the expression of TRAP220 mRNA and protein levels in temporal lobes from patients with chronic pharmacoresistant epilepsy who have undergone surgery.

Results: Expression of TRAP220 mRNA and protein was shown to be decreased significantly in the temporal cortex of the patients with epilepsy.

Conclusions: Our work showed that a decrease in TRAP220 mRNA and protein levels may be involved in the pathophysiology of epilepsy and may be associated with impairment of the brain caused by frequent seizures.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Blotting, Western
  • Child
  • Down-Regulation
  • Epilepsy / diagnosis
  • Epilepsy / genetics
  • Epilepsy / metabolism*
  • Female
  • Humans
  • Immunohistochemistry
  • Male
  • Mediator Complex Subunit 1
  • Middle Aged
  • RNA, Messenger / metabolism
  • Temporal Lobe / metabolism*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*

Substances

  • MED1 protein, human
  • Mediator Complex Subunit 1
  • RNA, Messenger
  • Transcription Factors