Terminal B cell differentiation is skewed by deregulated interleukin-6 secretion in beta2 integrin-deficient mice

J Leukoc Biol. 2006 Sep;80(3):599-607. doi: 10.1189/jlb.1205740. Epub 2006 Jul 14.

Abstract

Absence of the common beta chain (CD18) of beta(2) integrins leads to leukocyte-adhesion deficiency type-1 (LAD1) in humans. Mice with a CD18 null mutation suffer from recurrent bacterial infections, impaired wound healing, and skin ulcers, closely resembling human LAD1. Previous findings in CD18(-/-) mice demonstrated a skewed terminal B cell differentiation with plasmacytosis and elevated serum immunoglobulin G (IgG). As interleukin-6 (IL-6) is a potent enhancer of plasma cell formation and Ig secretion, we assessed IL-6 serum levels of CD18(-/-) and wild-type (WT) mice kept under a conventional or barrier facility or specific pathogen-free (SPF) conditions. We detected an up to 20-fold increase in IL-6 in serum of CD18(-/-) mice compared with WT controls when kept under conventional or barrier facility conditions, respectively. Under SPF conditions, no significant differences in terms of IL-6 serum levels were found between CD18(-/-) and WT mice. However, histological alterations of secondary lymphoid tissues, plasmacytosis, abnormal plasmacytoid cells (Mott cells), and hypergammaglobulinemia persisted. To further analyze the role of IL-6 in these pathological alterations, we established a CD18(-/-) IL-6(-/-) double-deficient mouse mutant. In these mice, serum IgG levels were normal, and the altered plasma cell phenotype, including Mott cells, was no longer detectable. The CD18(-/-) IL-6(-/-) double-deficient mouse model thus demonstrated that IL-6 is responsible for parts of the phenotype seen in the CD18(-/-) mouse mutants. It may be of interest to examine human leukocyte-adhesion deficiency type-1 patients closer and search for pathological changes possibly induced via overproduction of IL-6.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • B-Lymphocytes / immunology*
  • B-Lymphocytes / pathology
  • CD18 Antigens / genetics*
  • CD18 Antigens / immunology
  • CD18 Antigens / metabolism*
  • Cell Differentiation / immunology*
  • Down-Regulation / immunology
  • Immunoglobulin G / biosynthesis
  • Immunoglobulin G / blood
  • Interleukin-6 / biosynthesis
  • Interleukin-6 / blood
  • Interleukin-6 / metabolism*
  • Lipopolysaccharides / administration & dosage
  • Macrophages / immunology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout

Substances

  • CD18 Antigens
  • Immunoglobulin G
  • Interleukin-6
  • Lipopolysaccharides