We report on harmonic generation by budding yeast cells (Saccharomyces cerevisiae, 10(8) cells/ml) in response to sinusoidal electric fields with amplitudes ranging from zero to 5 V/cm in the frequency range 10-300 Hz. The cell-generated harmonics are found to exhibit strong amplitude and frequency dependence. Sodium metavanadate, an inhibitor of the proton pump known as H+-ATPase, and glucose, a substrate of H+-ATPase, are found to increase harmonic production at low amplitudes while reducing it at large amplitudes. This P-type proton pump can be driven by an oscillatory transmembrane potential, and its nonlinear response is believed to be largely responsible for harmonic production at low frequencies in yeast cells. We find that the observed harmonics show dramatic changes with time and in their field and frequency dependence after perturbing the system by adding an inhibitor, substrate, or membrane depolarizer to the cell suspension.