Abstract
The monoclonal antibody MOMA-1 is a widely-used marker for marginal metallophilic macrophages in spleen and some other subsets of macrophages. The antigen recognized by MOMA-1 has yet to be characterized, but its expression pattern is similar to that of sialoadhesin (Sn, CD169, Siglec-1), a member of the sialic acid binding Ig-like lectin (Siglec) family. Using flow cytometry of Sn-transfected cells and staining of lymphoid tissue sections from Sn-deficient mice, we demonstrate here that the antigen recognized by MOMA-1 is Sn.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Antibodies, Monoclonal / immunology*
-
Antigens / immunology*
-
CHO Cells
-
Cricetinae
-
Membrane Glycoproteins / genetics
-
Membrane Glycoproteins / immunology*
-
Membrane Glycoproteins / metabolism
-
Mice
-
Mice, Inbred C57BL
-
Receptors, Immunologic / genetics
-
Receptors, Immunologic / immunology*
-
Receptors, Immunologic / metabolism
-
Sialic Acid Binding Ig-like Lectin 1
Substances
-
Antibodies, Monoclonal
-
Antigens
-
Membrane Glycoproteins
-
Receptors, Immunologic
-
Sialic Acid Binding Ig-like Lectin 1
-
Siglec1 protein, mouse