Abstract
To rapidly discover clinically useful angiogenesis inhibitors, we created and screened a library of existing drugs for inhibition of endothelial cell proliferation. Mycophenolic acid (MPA), an immunosuppressive drug, was found to potently inhibit endothelial cell proliferation in vitro and block tumor-induced angiogenesis in vivo. Using RNA interference, we found that knockdown of one of the two known isoforms of inosine monophosphate dehydrogenase (IMPDH-1) is sufficient to cause endothelial cell cycle arrest.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Angiogenesis Inhibitors / chemistry*
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Angiogenesis Inhibitors / pharmacology*
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Animals
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Cell Proliferation / drug effects
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Cells, Cultured
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Enzyme Inhibitors / chemistry
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Enzyme Inhibitors / pharmacology
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Humans
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IMP Dehydrogenase / antagonists & inhibitors*
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IMP Dehydrogenase / genetics
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IMP Dehydrogenase / metabolism*
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Mice
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Molecular Structure
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Mycophenolic Acid / chemistry*
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Mycophenolic Acid / pharmacology*
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Neoplasms / blood supply
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Neoplasms / pathology
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RNA Interference
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Umbilical Cord / drug effects
Substances
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Angiogenesis Inhibitors
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Enzyme Inhibitors
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IMP Dehydrogenase
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Mycophenolic Acid