Western blot immunoassay as a confirmatory test for the presence of anti-Mycoplasma hyopneumoniae antibodies in swine serum

J Vet Diagn Invest. 2006 Mar;18(2):198-201. doi: 10.1177/104063870601800210.

Abstract

A Western blot immunoassay (WBI) was developed as a confirmatory test for 2 commercial Mycoplasma hyopneumoniae (Mhyo) ELISAs. The WBI detected at least 5 antigen bands (150, 130, 74, 70, and 30 kDa) from Mhyo whole membrane proteins that were not present in the antigens prepared from M. hyorhinis and M. hyosynoviae. Among discrepant sera from vaccinated pigs (n = 17) and field samples (n = 91) assayed by WBI: 1) 2 of the ELISA-positive samples reacted with all 5 antigens bands; 2) all blocking ELISA-positive samples (n = 53) bound 150-, 130-, 74-, and 70-kD antigen bands; and 3) all indirect ELISA-positive samples (n = 55) bound 150-, 130-, and 30-kD antigens. We conclude that the WBI targeting the top 4 antigen bands is a useful confirmatory test for samples initially screened using the commercial Mhyo ELISAs.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Antibodies, Bacterial / blood*
  • Blotting, Western / methods
  • Blotting, Western / veterinary*
  • Mycoplasma hyopneumoniae / immunology
  • Mycoplasma hyopneumoniae / isolation & purification*
  • Pneumonia of Swine, Mycoplasmal / diagnosis
  • Pneumonia of Swine, Mycoplasmal / microbiology*
  • Respiratory Tract Infections / diagnosis
  • Respiratory Tract Infections / microbiology
  • Respiratory Tract Infections / veterinary*
  • Swine

Substances

  • Antibodies, Bacterial