Microdevice DNA forensics by the simple tandem repeat method

J Chromatogr A. 2006 Apr 14;1111(2):206-13. doi: 10.1016/j.chroma.2005.05.100. Epub 2005 Jun 22.

Abstract

We review recent experiments on DNA forensics by the simple tandem repeat (STR) method using a 16-lane micromachined device as the active separation element. Separations by linear polyacrylamide matrices show very high data quality metrics when evaluated with statistically significant data sets. Full 16-locus multiplexes are verified on the multilane system. Multi-donor mixed samples are studied in the context of the limits of the laser-induced fluorescence detector and data-reduction software. The microdevice appears to be posed to outperform current capillary arrays in terms of stability and, through specialized sample loading, in the interpretation of complex mixtures.

Publication types

  • Review

MeSH terms

  • DNA / genetics*
  • Forensic Medicine*
  • Humans
  • Tandem Repeat Sequences*

Substances

  • DNA