HIV-1 envelope triggers polyclonal Ig class switch recombination through a CD40-independent mechanism involving BAFF and C-type lectin receptors

J Immunol. 2006 Apr 1;176(7):3931-41. doi: 10.4049/jimmunol.176.7.3931.

Abstract

Switching from IgM to IgG and IgA is essential for antiviral immunity and requires engagement of CD40 on B cells by CD40L on CD4(+) T cells. HIV-1 is thought to impair CD40-dependent production of protective IgG and IgA by inducing progressive loss of CD4(+) T cells. Paradoxically, this humoral immunodeficiency is associated with B cell hyperactivation and increased production of nonprotective IgG and IgA that are either nonspecific or specific for HIV-1 envelope glycoproteins, including gp120. Nonspecific and gp120-specific IgG and IgA are sensitive to antiretroviral therapy and remain sustained in infected individuals with very few CD4(+) T cells. One interpretation is that some HIV-1 Ags elicit IgG and IgA class switch DNA recombination (CSR) in a CD40-independent fashion. We show that a subset of B cells binds gp120 through mannose C-type lectin receptors (MCLRs). In the presence of gp120, MCLR-expressing B cells up-regulate the CSR-inducing enzyme, activation-induced cytidine deaminase, and undergo CSR from IgM to IgG and IgA. CSR is further enhanced by IL-4 or IL-10, whereas Ab secretion requires a B cell-activating factor of the TNF family. This CD40L-related molecule is produced by monocytes upon CD4, CCR5, and CXCR4 engagement by gp120 and cooperates with IL-4 and IL-10 to up-regulate MCLRs on B cells. Thus, gp120 may elicit polyclonal IgG and IgA responses by linking the innate and adaptive immune systems through the B cell-activating factor of the TNF family. Chronic activation of B cells through this CD40-independent pathway could impair protective T cell-dependent Ab responses by inducing immune exhaustion.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • B-Cell Activating Factor
  • B-Lymphocytes / drug effects
  • B-Lymphocytes / immunology
  • B-Lymphocytes / metabolism
  • CD4 Antigens / metabolism
  • CD40 Antigens / metabolism
  • Cells, Cultured
  • Cytokines / pharmacology
  • HIV Envelope Protein gp120 / immunology*
  • Humans
  • Immunoglobulin Class Switching / genetics*
  • Immunoglobulins / biosynthesis
  • Immunoglobulins / immunology
  • Lectins, C-Type / immunology
  • Lectins, C-Type / metabolism*
  • Lymphocyte Activation
  • Mannose / metabolism
  • Membrane Proteins / immunology
  • Membrane Proteins / metabolism*
  • Protein Binding
  • Receptors, Antigen, B-Cell / immunology
  • Receptors, CCR5 / metabolism
  • Receptors, CXCR4 / metabolism
  • Recombination, Genetic / genetics*
  • Spleen / immunology
  • Spleen / metabolism
  • Tumor Necrosis Factor-alpha / immunology
  • Tumor Necrosis Factor-alpha / metabolism*
  • Up-Regulation

Substances

  • B-Cell Activating Factor
  • CD4 Antigens
  • CD40 Antigens
  • Cytokines
  • HIV Envelope Protein gp120
  • Immunoglobulins
  • Lectins, C-Type
  • Membrane Proteins
  • Receptors, Antigen, B-Cell
  • Receptors, CCR5
  • Receptors, CXCR4
  • TNFSF13B protein, human
  • Tumor Necrosis Factor-alpha
  • Mannose