Association between apolipoprotein E polymorphism and coronary artery disease in the Kermanshah population in Iran

Clin Biochem. 2006 Jun;39(6):613-6. doi: 10.1016/j.clinbiochem.2006.02.002. Epub 2006 Mar 20.

Abstract

Objective: Coronary artery disease (CAD) is the major cause of death in developing countries, such as Iran. The apolipoprotein E gene (APOE) is considered an important genetic determinant of CAD. In this study, the relationship between APOE polymorphism with lipid variation in CAD patients in Kermanshah, Iran was investigated.

Methods: This case-control study consisted of 115 CAD patients who angiographically had at least 30% stenosis and 135 unrelated controls. APOE polymorphism was detected by PCR-RFLP and serum lipid level was measured enzymatically.

Results: The APOE-epsilon4 and -epsilon2 allele frequencies were significantly higher in the CAD patients than in the control group (P < 0.001). The CAD patients with epsilon3/epsilon4 genotype had also higher TC (P < 0.001) and LDL-C (P < 0.01) and lower HDL-C (P < 0.03) levels than that of the control group.

Conclusions: APOE-epsilon4 allele is a risk factor for CAD, so that carriers of this allele with high levels of LDL-C may be susceptible to CAD and myocardial infarction.

MeSH terms

  • Apolipoproteins E / genetics*
  • Base Sequence
  • Coronary Artery Disease / epidemiology
  • Coronary Artery Disease / genetics*
  • DNA Primers
  • Female
  • Genetics, Population*
  • Humans
  • Iran / epidemiology
  • Male
  • Middle Aged
  • Polymerase Chain Reaction
  • Polymorphism, Genetic*
  • Polymorphism, Restriction Fragment Length

Substances

  • Apolipoproteins E
  • DNA Primers