[Relation between mutant p53 and multidrug resistance in gastric cancer]

Zhejiang Da Xue Xue Bao Yi Xue Ban. 2006 Jan;35(1):91-8. doi: 10.3785/j.issn.1008-9292.2006.01.017.
[Article in Chinese]

Abstract

Objective: To explore the relationship between mutant p53 and multidrug resistance in gastric cancer.

Methods: Mutant p53 (mp53) and mp53+sv40Tag were transferred to gastric cancer cell line SGC-7901. The MDR-1 mRNA was examined using RT-PCR, and the difference in chemotherapeutic sensitivity of SGC-7901 cells with mutant p53 was compared with those with mp53+sv40Tag and controls by MTT method.

Results: SGC-7901 cells with mutant p53 showed higher MDR-1 mRNA than that of other two groups. SGC-7901 cells with mutant p53 showed higher chemotherapeutic sensitivity to 5-Fu than that with mp53+sv40Tag and control (P<0.05), but no difference between those with mp53+sv40Tag and control (P>0.05). SGC-7901 cells with mutant p53 and those with mp53+sv40Tag showed higher chemotherapeutic sensitivity to ADM than control (P<0.05), but no difference between those with mp53 and with mp53+sv40Tag (P>0.05). There was no difference in chemotherapeutic sensitivity of SGC-7901 cells with mutant p53 compared with those with mp53+sv40Tag and control to CDDP (P>0.05).

Conclusion: Mutante p53 genes relates to multidrug resistance of gastric cancer.

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / biosynthesis*
  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / genetics
  • Adenocarcinoma / genetics
  • Drug Resistance, Multiple / genetics*
  • Humans
  • Mutation*
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Stomach Neoplasms / genetics*
  • Tumor Cells, Cultured
  • Tumor Suppressor Protein p53 / genetics*

Substances

  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • RNA, Messenger
  • Tumor Suppressor Protein p53